Work

The investigation of the relative levels of gene expression in the model cell lines, as well as the search for differentially expressed genes in the cell lines of human prostate adenocarcinomas with different ability to invasiveness and metastasis was done. Twelve differentially expressed genes between androgen-dependent cell line LNCaP and androgen-independent cell lines DU145 and PC3 were found. These genes may be potential markers for transition to androgen-independent tumor type. Moreover, fourteen genes with altered gene expression levels in PC3 cell line compared to the LNCaP cell line were observed. They might be potential markers of prostate cancer invasiveness and metastasis. The presence of genetic and epigenetic changes in different types of prostate tumors was investigated. Fourteen genes with a statistically significant difference in the frequency of deletions and methylation in different histological types of prostate tumors were obtained. All of the identified genes could be used for further research and for the creation of diagnostic panels for various types of prostate tumors.