Work

The aim of this work is to determine the angiogenic potential of placental mesenchymal stem cell-derived clones. The main idea of the work is to create a targeted therapeutic product - a cell population that secretes a defined spectrum of growth factors, cytokines that activate angiogenesis and vascularization, in order to correct pathological conditions and diseases associated with circulatory disorders. Since MSCs isolated from the placenta are heterogeneous cell culture in their morphological, biochemical and functional properties (Wen Jing Du, 2016), it is necessary to develop a technique for enriching the bulk MSCs with high angiogenic potential cells. For selected cultures, which are characterized by a high level of expression of genes associated with vascular development, conduct functional studies to assess the angiogenic potential in vitro. Cell cultures in the presence of which endotheliocytes form capillary-like structures should be selected as capable of activating angiogenesis.