Work

In order to study the potential antiresorptive and regulatory effects of newly synthesized pyrazole-containing bisphosphonates using in vitro models the following works will be performed: 1. Study of pro- and antioxidant properties of newly synthesized pyrazole-containing bisphosphonates in vitro using sensitive chromogenic substrates of 2,2-diphenyl- 1-picrylhydrazyl (DPPH), Dichlorophenolindophenol (DCIP) and TBA. 2. Study of the effect of new pyrazole-containing bisphosphonates on survival / viability monocyte-macrophage cell line J774A.1 or RAW264.7 using the MTT method. 3. Study of the influence of promising pyrazole-containing bisphosphonates on survival / viability primary culture of hepatocytes using the MTT method. 4. Research of isoprenoids formation in the mevalonate pathway under the influence of new pyrazole-containing bisphosphonates by using radiolabeled acetate (C14) on the models of monocyte-macrophage cell line J774A.1 or RAW264.7. 5. Research of cholesterol formation in the mevalonate pathway under the influence of new pyrazole-containing bisphosphonates by using radiolabeled acetate (C14) on the models of monocyte-macrophage cell line J774A.1 or RAW264.7 6. Study of the intensity of apoptosis in the monocyte-macrophage cell line J774A.1 or RAW264.7 under the action of pyrazole-containing bisphosphonates using Annexin V or TUNEL method. 7. Study of the regulatory effect of pyrazole-containing bisphosphonates on the mRNA expression of tranc_ription factor NFATc1, carbonic anhydrase 2 and IL-1β in a monocyte-macrophage cell line J774A.1 or RAW264.7 using RT-PCR.