Work

The work is devoted to the development of new QSAR models of biocidal activity and the targeted synthesis of sulfonamide derivatives of the isoxazole series with predicted properties. The literature data on the biological properties of sulfonamide derivatives of the isoxazole series will be analyzed and new original OSAR models will be developed using the OCHEM online platform . On the basis of the systematized information the theoretical and practical possibilities of obtaining the target isoxazole-containing sulfanilamides will be analyzed, convenient strategies of synthesis will be developed with the use ring closing metathesis reactions at the key stages. As a result, a number of preparative methods for obtaining promising isoxazole sulfanilamides will be developed and optimized. Compounds with predicted high antimicrobial activity will be synthesized and characterized by elemental analysis, NMR and mass spectroscopy. Target compounds with predicted high activity will be subjected to in vitro evaluation of antimicrobial potential against standard bacterial (Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Acinetobacter baumannii) and fungal (fungi of the genus Candida) method on a solid nutrient medium (Mueller-Hinton and Saburo agar). The antioxidant properties of the synthesized derivatives will be determined by the percentage of malonic dialdehyde as the final product of the process of ascorbate-dependent free radical lipid peroxidation. Toxicological evaluation of the most promising sulfonamide derivatives will be carried out according to generally accepted European standards (OECD 202) using the aquatic D. magna as a known biosensor of environmental safety of biologically active compounds and determine the toxicity class of compounds according to D.R. Passino with co-authors.