Work

The aim of this project is to develop a system for positive selection of ethanol producers based on the inducible promoter of the alcA gene of the fungus Aspergillus nidulans and the trancription factor alcR, which binds to this promoter in the presence of ethanol. This idea will be realized through the use of molecular genetic methods for the construction of strains of yeasts Saccharomyces cerevisiae, Ogataea polymorpha and Komagataella phaffii, in which the expression of genes LAC4 Kluyveromyces lactis, kanMX4 or kanMX4mod under the control of ethanol-induced alcA promoter will be combined with the expression of the gene alcR A. nidulans. The engineered recombinant strains of yeasts S. cerevisiae, O. polymorpha and K. phaffii will be used for selection of UV- or insertion mutants with altered levels of ethanol production during fermentation of various Carbon sources (glucose, glycerol, xylose, etc.), which will allow us to identify genes that affect the efficiency of alcoholic fermentation in these yeasts.