Work

Only few effective protocols have been developed for the genetic transformation of a number of important cultural plants, in particular soybeans, wheat, grapes and many monocotyledons. Existing protocols are rather complicated, labor-intensive and expensive because they often involve working with embryonic tissues, cultivating and regenerating an adult plant in vitro. Our work is based on the idea of artificial induction of double-stranded DNA breaks by the action af stressful factors to increase directly or indirectly the number of available sites for the integration and activation of reparative cell systems that are involved in the integration of T-DNA or other DNA vectors into the host genome. Іn accordance with the foregoing the aim of the project is development of an effective, reliable, simple, inexpensive method of genetic transformation of plants using stress factors that induced double-stranded breaks in the host cell DNA. Increasing the activity of DNA repair systems through the use of inducing doses/concentrations of stress factors should affect the effectiveness of genetic transformation, which ensures the realization of the aim. As a result of the project, it is expected to develop an effective, reliable, simple, inexpensive method of genetic transformation based on existing protocols, using stress factors that will simplify and accelerate the process of genetic transformation of plants. The significance of the work lies in the fact that the development and implementation of highly effective, simple, cheap protocols of genetic transformation using stress factors will allow producing plants with the necessary properties to be much cheaper, as well as increase the efficiency of genetic transformation of important cultivated plants.