Work

The aim of the work is to obtain effective producers of lactic acid from renewable raw materials (lignocellulose) based on the yeasts Ogataea polymorpha and Scheffersomyces stipitis. To achieve this goal, recombinant strains of yeasts O. polymorpha and S. stipitis with the expression of genes encoding the enzyme lactate dehydrogenase from different sources (cattle, micellar fungi, lactic acid bacteria, etc) will be obtained, and the ability of these strains to produce lactic acid from glucose and xylose (the main components of lignocellulosic hydrolysates) will be tested. Additionally, the selection of strains resistant to high lactic acid content in the culture medium will be carried out, as well as the optimization of cultivation conditions for maximum yield of the final product.