Work

The idea of the research is to develop a method of the synthesis and to obtain a structure of dioximate iron (II) clathrochelate complex that will be structurally close to the anti-cancer agent and tagged with a fluorescent functional group for study its accumulation in cancer cells. Thus, the aim of the project is to develop a method for the synthesis and preparation of the fluorescently tagged tris-dioximate iron (II) clathrochelate complexes and to examine their properties and interactions with proteins, as well as biological activity in cancer cells. To achieve this goal, we are planning to develop the methods for targeted modification of iron (II) clathrochelate complexes; to synthesize a number of iron (II) clatrochelates functionalized by linker groups - precursors of fluorescently tagged complexes; to develop a method of modification of linker groups by fluorescent tags. Then we are planning to characterize the properties of synthesized iron (II) clathrochelates and to study their interaction with globular proteins, in particular albumins; to investigate the potential biological activity of the iron (II) clatrochelates in cancer cells, to investigate their distribution and accumulation in cancer cells). As a result, we are going to obtain a method for the selective modification of the iron (II) clathrochelate complexes and fluorescently tagged iron (II) clathrochelates for use in biological systems. In the future, the known mechanism of anticancer activity of iron (II) clathrochelate complexes may be the important tool for the development of agents to prevention and treatment of oncological diseases.